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By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

The seriously acclaimed laboratory common, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really a vital ebook for researchers in all fields of existence sciences

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H. Reed and S. D. Morgan, Biochemistry 13, 3537 (1974). • K. D. Collins and G. R. Stark, J. Biol. Chem. 246, 6599 (1971). h W. B. Dempsey and E. E. Snell, Biochemistry 2, 1414 (1973). G. E. Lienhard and I. I. Secemski, J. Biol. Chem. 246, 1121 (1973). i E. J. Milner-White and D. C. Watts, Biochem. J. 122, 727 (1971). k D. H. Buttlaire and M. Cohn, J. Biol. Chem. 249, 5733 (1974). K. A. Koetfler and G. P. Hess, Biochemistry 18, 5345 (1974). K. A. Koehler and G. E. Lienhard, Biochemistry 10, 2477 (1971).

In general, acid proteases seem to be quite sensitive to inhibition by diazo compounds, although cupric ion may be required for a facile reaction. 1~ Recently, fl-galactosidase has been reported to be irreversibly inhibited by the diazo sugar 2,6-anhydro-l-diazo-l-deoxy-D-glycero-L-manno-heptitel. ~ Protonation of the diazo group to yield the highly reactive diazonium ion is thought to mediate the inhibition. These specific reagents have not been sufficiently exploited and could be used fruitfully with many different kinds of enzymes in which acid catalysis occurs.

Lai and B. Axelrod, Biochem. Biophys. Res. Commun. 64, 463 (1973). B. E. Evans and R. Wolfenden, J. Am. Chem. Soc. 92, 4751 (1970). P. W. K. Woo, It. W. Dion, S. M. Lange, L. F. Dahl, and L. J. Durham, J. Helerocycl. Chem. 11, 641 (1974). R. M. Cohen and R. Wolfenden, J. Biol. Chem. 246, 7561 (1971). L. D. Byers and R. Wolfenden, J. Biol. Chem. 247, 606 (1972). A. Schmitt, I. Bottke, and G. Siebert, Hoppe-Seyler's Z. Physiol. Chem. 347, 18 (1966). "M. Wishnick, M. D. Lane, and M. C. Scrutton, J.

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