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By Geoffrey H. Bourne

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Bourne, Nature 133, 874 (1933). 101 G. H. Bourne, Australian J. Exptl. Biol. Med. Sei. 11, 261 (1933). 102 G. H. Bourne, Nature 132, 859 (1934). 104 The principle of the technique is based upon the fact that the vitamin is a powerful reducing agent and that it will reduce silver nitrate not only in alkaline solution, as many other biological substancet do, but also in neutral and acid solution. The first preparations by Bourne100·101 were made with neutral silver nitrate. First attempts to demonstrate the vitamin were made on the adrenals (which are extremely rich in the vitamin) of cat and guinea pig which had been fixed in the vapor of formaldehyde.

Skin 2. Alimentary T r a c t , Nervous System, and Bone 3. Adrenals 4. Embryos Folic Acid Deficiency Vitamin C Deficiency 1. General Background 2. Clinical Scurvy 3. Experimental Scurvy 4. Effect of Scurvy on Repair of Tissues 5. Estrogens and Bone Growth in Scurvy 6. Vitamin C and Collagen 7. Vitamin C and Calcification 8. Reproductive Organs Vitamin D Deficiency 1. Rickets 2. Bone Changes 3. T e e t h 4. Bone Repair 5. Soft Tissues Vitamin E Deficiency 1. General Description 2. E m b r y o 3.

Single neurone from the hypoglossal nucleus of rat brain, showing localization of an enzyme splitting adenosine triphosphate. Nucleolus strongly positive and cytoplasm lightly positive. 5. A, B, C, D, E, F, G, and / are by courtesy of D . Naidoo and O. E. P r a t t and the Journal of Neurology, Neurosurgery and Psychiatry. INTRACELLULAR LOCALIZATION OF ENZYMES AND VITAMINS •V·" ", " v D 35 - v Ά ';; ^ίΚ,: ;--: 36 GEOFFREY H. PLATE BOURNE III A. Simple esterase in the nasal mucosa of the rabbit.

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