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Ammonia and Nitrate as N Sources 85 85 85 86 87 87 88 88 89 90 90 91 92 THE PHYTOFLAGELLATES VIII. Growth Factors and Chemical Asepsis A. Euglena and Vitamin B^ 1. Historical 2. Specificity of B u 3. Ecological Aspects of Vitamin B ^ 4. Is a B12 Requirement a Phyletic Character in Euglenids? . . 5. The Minimal Organic Requirement of Euglenids B. Chlamydomonas chlamydogama : Vitamin Β ω , Histidine, and Aspartic Acid Requirements C. Growth Factor Requirements for Other Phytoflagellates 1. Growth Factors and Isolation Procedures 2.

Pasteur 76, 497. Treboux, O. (1903). Flora 92, 49. von Brand, T. (1935). Ergeh. Biol. 12, 161. Van Niel, C. B. (1949). In Photosynthesis in P l a n t s , j ) p . 437-495. J . Franck and W. E. , The Iowa State College Press, Ames, Iowa. Winogradsky, S. (1888). Beiträge zur Morphologie und Physiologie der Bakterien. I. Zur Morphologie und Physiologie der Schwefelbakterien. Arthur Felix, Leipzig. The Phytoflagellates S. H. HUTNEB AND LUIGI PKOVASOLI Baskins Laboratories, New York It is better to ask some of the questions than to know all the answers.

C4 + Cx Addition : Glutamate 6. Other Carboxylation Reactions D. Glucose Utilization and Phosphorylations The Glucose Block E. Higher Alcohols and Higher F a t t y Acids F. Glycine G. Acetate Flagellates and the Origin of the Protozoa 54 54 56 56 58 60 60 60 63 64 66 68 70 73 74 74 74 75 77 77 V. Streptomycin-Induced Apochlorosis A. Historical and General B. Specificity of Streptomycin C. Mode of Action of Streptomycin 1. The Nucleic Acid Theory 2. The Chlorophyll Interference Theory 3. The Condensation-Reaction Theory of Umbreit et al 4.

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