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By José M. Musacchio (auth.), Leslie L. Iversen, Susan D. Iversen, Solomon H. Snyder (eds.)

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1959, Inhibition of O-methylation of epinephrine and norepinephrine in vitro and in vivo, Seienee 130:800. , 1958, Enzymatic O-methylation of catecholamines, J. Biol. 233:702-705. , and VESELL, E. , 1970, Heterogeneity of N- and O-methyltransferases, Mol. Pharmacol. 6:78-84. , and CLEMENTE, C. , 1959, Distribution of catechol-Omethyltransferase in the nervous system and other tissues, J. Neuroehem. 5:68-72. , 1968, Inhibition of tyrosine hydroxylase by aquayamycin, J. Antibiot. 21:350-355. , 1969, Studies on a oew pigment antibiotic chrothiomycin, J.

Neurochem. 20:799-805. COLLINS, G. G. , WILLIAMS, E. , and YOUDIM, M. B. , 1970, Multiple forms of human brain mitochondrial monoamine oxidase, Nature 255:817-820. CONNETT, R. , 1970, Purification and properties of bovine phenylethanolamine N-methyltransferase,]. Biol. Ghem. 245:329-334. 28 lOSE. M. , GREEN,A. , KOSLOW, S. , LEFEVRE, H. , REVUELTA, A. , 1972, Dopamine and norepinephrine in noradrenergic axons: A study in vivo of their precursor product relationship by mass fragmentography and radiochemistry, Pharmacol.

Two groups of reticular nerve cell bodies have been localized in the medulla oblongata. The PNMT-containing terminals are mainly found in certain nuclei of the lower brain stern, in the locus coeruleus, in certain nuclei of the hypothalamus, and in the periventricular gray matter. , 1974). 1. Assay The principle of the radiometrie assay was originally developed by Axelrod (1962a) and it is based on the use of 5-[ methyl- 14 C]adenosyl-Lmethionine (for details, see Coyle, Chap. 4, Vol. 1). 2. Purification PNMT was partially purified by Axelrod (1962a).

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