By T. Sreevalsan (auth.), Ronald L. Cihlar, Richard A. Calderone (eds.)
Over the process the prior decade, there were outstanding advances within the examine of human pathogenic fungi. those advancements have taken position all through a variety of disciplines, and feature come because the results of newly to be had genome sequences of pathogens reminiscent of candida albicans and different version fungi. In Candida Albicans: tools and Protocols, specialist researchers discover those intriguing new insights, targeting the research of medically very important fungi and Candida spp particularly. Chapters research serious points of molecular tools, supplying info on reporter gene assays, transformation, gene expression in vivo, and strategies for large-scale gene disruption. while, the paintings contains in-depth descriptions of illness versions of candidiaisis, evidence approximately pressure id, and guidance at the guidance of samples for proteomic investigations and tandem affinity purification. Composed within the hugely profitable Methods in Molecular Biology™ sequence structure, every one bankruptcy encompasses a short advent, step by step tools, an inventory of worthy fabrics, and a Notes part which stocks pointers on troubleshooting and fending off recognized pitfalls.
Authoritative and leading edge, Candida Albicans: tools and Protocols is a useful resource of equipment for investigators within the exhilarating fields of clinical and molecular mycology.
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Additional resources for Candida albicans: Methods and Protocols
6. E. (2005) Candida species. ), 6th Ed, Churchill Livingstone, Philadelphia, pp. 2938–2956. 7. Segal, E. and Elad, D. (2005). Candidiasis. ), 10th Ed, Arnold, London, pp. 579–623. 8. , and Eylan, E. (1981) Protective immunity against murine candidiasis elicited by Candida albicans ribosomal fractions. Infect. Immun. 31, 874–878. 9. Segal, E. and Sandovsky-Losica, H. (1981) Experimental vaccination with Candida 10. 11. 12. 13. 14. 15. 16. 17. albicans ribosomes in cyclophosphamide treated animals.
Sterile polycarbonate membrane filters (diameter 25 mm) are placed on the surface of YNB agar containing 50 mM glucose (see Note 3). 2. A standardized cell suspension (50 mL) is applied to the surface of each membrane and the plates are incubated at 37°C. 3. After 24 h, the membrane-supported biofilms are transferred to fresh agar for a further 24 h, giving a total incubation time of 48 h for biofilm formation. 3. Penetration of Biofilms by Antifungal Agents 1. After biofilm formation on membrane filters, smaller sterile polycarbonate membrane filters (diameter 13 mm) are carefully placed on top of the 48-h-old biofilms.
The present chapter describes the artificial throat model, a model system for growing mixed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent in situ hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms. Key words: Artificial throat, shunt prosthesis, mixed-species biofilm, microbial interactions, fluorescent in situ hybridization, FISH. 1. 1. Biofilms and Infection Many microbial infections can be linked to the ability of the infectious agents to establish biofilms (1), and thus, biofilm formation is of concern in clinical settings.
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